Physiology

Renal Physiology

GFR, tubular transport, RAAS, acid-base balance, electrolytes, and renal hormones

GFR and Glomerular Filtration

Normal GFR: approximately 120 mL/min (180 L/day filtered; about 1.5 L/day excreted). Governed by Starling forces across the glomerular capillary.

FactorEffect on GFRClinical Example
Afferent arteriole dilationIncreases GFRProstaglandins, ANP
Afferent arteriole constrictionDecreases GFRNSAIDs, severe hypotension
Efferent arteriole constrictionIncreases GFR (initially)Angiotensin II
Efferent arteriole dilationDecreases GFRACE inhibitors and ARBs
⭐ ACE Inhibitors and KidneyACEi/ARBs dilate EFFERENT arteriole → decrease GFR → expected creatinine rise (up to 30% acceptable). Avoid in bilateral renal artery stenosis — both kidneys depend on Ang II to maintain GFR.

Tubular Reabsorption and Diuretics

SegmentWhat is ReabsorbedDiuretic Target
Proximal tubule67% Na+, water, HCO3-, glucose, amino acids, phosphate, uric acidCarbonic anhydrase inhibitors (acetazolamide)
Loop of Henle (thick ascending)Na+/K+/2Cl- (25% of Na+); impermeable to waterLoop diuretics (furosemide) — NKCC2 cotransporter
Distal convoluted tubuleNa+/Cl- (5-8%); Ca2+ (PTH-stimulated)Thiazides (NCC cotransporter)
Collecting ductNa+ (aldosterone); water (ADH/vasopressin)K+-sparing diuretics (spironolactone, amiloride)
🧐 Diuretics by LocationProximal=Acetazolamide. Loop=Furosemide (NKCC2). DCT=Thiazides (NCC). Collecting Duct=Spironolactone (aldosterone antagonist). Furosemide is most potent — acts on thick ascending limb where 25% of Na+ reabsorption occurs.

Renin-Angiotensin-Aldosterone System

Renin (JGA) → cleaves angiotensinogen to Ang I → ACE (lungs) converts to Ang II → aldosterone (adrenal cortex) + vasoconstriction + ADH release + thirst.

🩹 Aldosterone EffectsCollecting duct: inserts ENaC channels and Na+/K+-ATPase → Na+ reabsorption, K+ and H+ secretion. Net: Na+ and water retention + hypokalemia + metabolic alkalosis. Primary hyperaldosteronism (Conn's): HTN + hypokalemia + metabolic alkalosis.

Acid-Base Physiology

DisorderpHPrimary ChangeCompensation
Metabolic acidosisLess than 7.35HCO3- decreasedHyperventilation (Winter's formula: expected PaCO2 = 1.5 x HCO3 + 8 +/- 2)
Metabolic alkalosisGreater than 7.45HCO3- increasedHypoventilation (raise PaCO2)
Respiratory acidosisLess than 7.35PaCO2 increasedKidneys retain HCO3- (days)
Respiratory alkalosisGreater than 7.45PaCO2 decreasedKidneys excrete HCO3-
🧐 High Anion Gap Metabolic Acidosis — MUDPILESMethanol, Uremia, DKA/starvation ketosis, Propylene glycol, Isoniazid/Iron, Lactic acidosis, Ethylene glycol, Salicylates.

Key Electrolytes

ElectrolyteNormalHyperstate SignsHypostate Signs
Sodium135-145 mEq/LHypernatremia: thirst, confusion, seizureHyponatremia: headache, nausea, seizure (severe)
Potassium3.5-5.0 mEq/LPeaked T, wide QRS, fatal arrhythmiaFlat T waves, U waves, weakness, cramps
Calcium8.5-10.5 mg/dLBones stones groans moans; shortened QTChvostek/Trousseau; prolonged QT; tetany
Magnesium1.5-2.5 mEq/LLoss of DTRs, respiratory depressionRefractory hypokalemia; torsades de pointes

Renal Hormones

  • Erythropoietin (EPO) — peritubular fibroblasts; stimulates RBC production; deficient in CKD → normocytic anemia
  • Renin — JGA cells; triggers RAAS; released by decreased renal perfusion, low Na+ delivery, or sympathetic stimulation
  • 1,25-dihydroxyvitamin D (calcitriol) — 1-alpha-hydroxylase in kidney converts 25-OH-D3 to active form; promotes Ca2+ and PO4 absorption; deficient in CKD → renal osteodystrophy
  • Prostaglandins (PGE2, PGI2) — dilate afferent arteriole to maintain GFR when BP falls; NSAIDs block → AKI in dehydration/low-flow states

Clinical Pearls

⭐ ADH and SIADH vs DIADH (vasopressin): released with high serum osmolarity or low blood volume → V2 receptors in collecting duct → aquaporin-2 insertion → water reabsorption. SIADH: excess ADH → hyponatremia + euvolemia + urine osmolarity greater than 100. Central DI: no ADH → dilute urine + hypernatremia + treats with desmopressin (DDAVP). Nephrogenic DI: ADH present but receptor insensitive → treats with low-Na diet + thiazides.
🩹 FENa InterpretationFENa = (urine Na x plasma Cr) / (plasma Na x urine Cr) x 100. Less than 1% = prerenal. Greater than 2% = intrinsic renal (ATN). Unreliable on diuretics — use FEUrea instead (less than 35% = prerenal).