Physiology

Pancreatic Physiology

Exocrine enzyme secretion, endocrine glucose regulation, and hormonal feedback

Exocrine Pancreatic Function

The exocrine pancreas produces ~1.5 L/day of bicarbonate-rich juice and digestive enzymes, secreted into the pancreatic duct → duodenum. Regulated by CCK and secretin.

EnzymeForm SecretedActivated BySubstrate
TrypsinogenZymogen (inactive)Enterokinase (brush border)Proteins (endopeptidase)
ChymotrypsinogenZymogenTrypsinAromatic amino acids
ElastaseZymogenTrypsinElastin and connective tissue proteins
Pancreatic lipaseActiveTriglycerides (requires colipase and bile salts)
Pancreatic amylaseActiveStarch (polysaccharides)
BicarbonateSecreted by ductal cellsSecretinNeutralizes gastric acid in duodenum (optimal pH for enzymes)
🩹 Pancreatitis MechanismPremature activation of trypsinogen within pancreas → autodigestion. Causes: gallstones (most common in women), alcohol (most common in men), hypertriglyceridemia, hypercalcemia, ERCP, medications, trauma, idiopathic. Trypsin activates all other zymogens → cascade damage.

Endocrine Pancreas — Islets of Langerhans

Cell TypePercentageHormoneStimulus
Beta cells70%Insulin, C-peptide, amylinGlucose, amino acids, GIP, GLP-1, vagal (ACh)
Alpha cells20%GlucagonHypoglycemia, amino acids, stress, fasting
Delta cells5%SomatostatinGlucose, fatty acids, GLP-1 — inhibits alpha and beta cells
PP cells5%Pancreatic polypeptideProtein; inhibits pancreatic exocrine secretion

Insulin Physiology

Insulin is the primary anabolic hormone. Released in biphasic pattern: first phase (preformed insulin, within 2-5 min of glucose) and second phase (new insulin synthesis, over 30-60 min).

TargetInsulin Effect
LiverGlycogenesis, glycolysis, lipogenesis; inhibits gluconeogenesis and glycogenolysis
MuscleGlucose uptake (GLUT4), glycogenesis, protein synthesis
AdiposeGlucose uptake (GLUT4), lipogenesis (triglyceride storage), inhibits lipolysis and HSL
ElectrolytesDrives K+ into cells (used in hyperkalemia treatment); also Mg2+ and PO4-
⭐ C-Peptide Clinical UseC-peptide is co-secreted with insulin in equimolar amounts. Low C-peptide + high insulin = exogenous insulin injection (malingering/factitious). High C-peptide + high insulin = insulinoma. Used to distinguish endogenous vs. exogenous hyperinsulinism.

Glucagon and Counter-Regulatory Hormones

HormoneSourceEffect on Blood GlucoseKey Mechanism
GlucagonAlpha cellsRaisesHepatic glycogenolysis + gluconeogenesis; lipolysis; ketogenesis
EpinephrineAdrenal medullaRaisesGlycogenolysis (liver + muscle); lipolysis; inhibits insulin secretion (alpha-2)
CortisolAdrenal cortexRaisesGluconeogenesis; insulin resistance; protein catabolism
Growth hormoneAnterior pituitaryRaisesInsulin resistance; lipolysis; "dawn phenomenon"
🧐 Counter-Regulatory Hormones"GECC" — Glucagon, Epinephrine, Cortisol, GH — all raise blood glucose. All are catabolic except GH (anabolic in other tissues). Deficiency of all four = profound hypoglycemia (as in adrenal insufficiency).

Clinical Pearls

⭐ Incretin EffectOral glucose raises insulin more than IV glucose (same dose) — the "incretin effect." GIP (gastric inhibitory peptide) from K cells and GLP-1 from L cells are released with oral nutrients → potentiate glucose-stimulated insulin secretion. GLP-1 agonists (semaglutide, liraglutide) and DPP-4 inhibitors exploit this mechanism.
🩹 Whipple's TriadCriteria for insulinoma (or any hypoglycemia disorder): (1) symptoms of hypoglycemia, (2) low blood glucose during symptoms, (3) relief with glucose administration. Insulinoma: most common pancreatic endocrine tumor; usually benign, solitary; diagnosed with 72-hour fast + insulin and C-peptide levels.